At lower concentrations, trophozoites and schizones persisted with defects in morphological conditions. Cytotoxic activity was determined against mrc5 cells to assess selectivity. In vitro antiplasmodial activity of crude extracts of. In the in vivo evaluation of the antiplasmodial activity of the plant extracts was employed against chloroquine cq sensitive p. In vivo antimalarial activity of extracts of tanzanian medicinal plants used for the treatment of malaria. Leishmanicidal activity of lipophilic extracts of some hypericum species. Health, general biological sciences science and technology, general antiparasitic agents research medical research medicinal plants medicine, experimental parasitic diseases st. Crude extracts of some parts of garcinia parvifolia miq.
Antiplasmodial activity of extracts of selected medicinal plants used by local communities in western uganda for treatment of malaria. Research article evaluation of the in vitro antiplasmodial. Pdf antiplasmodial activity of extracts of selected. The antiplasmodial activity was quantified by the lactate dehydrogenase assay used by mesia et al. Antiplasmodial activity of some medicinal plants used in. Norcaesalpin d was isolated by column chromatography from dichloromethane root extract of caesalpinia bonducella and its structure was assigned based on the spectral data. Pdf antiplasmodial, antitrypanosomal, antileishmanial. Antiplasmodial effect of plant extracts from picrolemma huberi and. The chemical injury to erythrocytes was also carried out and it showed that there were no morphological changes in erythrocytes by the ethanolic extract of all the tested plant extracts. Read antiplasmodial activity of extracts and quassinoids isolated from seedlings of ailanthus altissima simaroubaceae, phytotherapy research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Six methanol meoh extracts and one chloroform extract showed in vitro antiplasmodial activity against the d6 plasmodium falciparum strain, while only three. Crude extracts from leaves, bark and fruits were tested for their antiplasmodial activity against the chloroquinesensitive strain of plasmodium falciparumd10, where leaf extracts showed the highest activity. Antiplasmodial, antiinflammatory and cytotoxic activities. Three plant extracts caused 100% inhibition of the parasite growth at concentrations of plant material.
Flavonoids have been suggested as candidates for increasing antiplasmodial activity and potentially slowing the emergence of resistance in wholeplant preparations, relative to artemisinin alone weathers et al. In this study, the antiplasmodial activities of 11 medicinal plants. Flavonoids had no effect on the in vitro antiplasmodial activity of a. In vitro evaluation of antiplasmodial activity of extracts.
The extracts were then tested for antiplasmodial activity either as a fresh, frozen. Antimicrobial, antiplasmodial, haemolytic and antioxidant activities of. The antiplasmodial activities of the ether and methanol extracts were expressed as 50% effective concentration ec50 that was determined from doseresponse curve by nonlinear regression analysis. Pdf antiplasmodial activity of aqueous leaf extract of. Against the scourge of malaria in africa and the development of chemoresistance, discovering new natural cures is a major concern for many researchers.
Antimicrobial, antiplasmodial, haemolytic and antioxidant. The results of the in vitro antiplasmodial activity of extracts are presented in table i. Tml and tmr showed in vitro antiplasmodial activity against fcb1 strain of p. Ten extracts and fractions from various plant parts of calotropis procera and gumoleo resin of commiphora wightii were chosen from a total of 35 extracts based on their ranked in vitro schizontocidal activity with p. Elisa hrp2 assay was used to evaluate the in vitro inhibitory. Antiplasmodial activity and phytochemical analysis of extracts from selected ugandan medicinal plants article pdf available in journal of ethnopharmacology 186 march 2016 with 710 reads. Larvicidal, antipyretic and antiplasmodial activity of. Pdf in vitro antiplasmodial activity of crude extracts. In vivo antiplasmodial activity of two sahelian plant. Screening of plants from new caledonia for antiplasmodial activity against plasmodium falciparum revealed that methanolic extracts of the leaves and bark of tristaniopsis calobuxus, t. Medicinal plants, a validated source of new medicines, have been used.
Pdf global reporting of 5 million more malaria cases in 2016 comparative to 211 million cases reported in 2015. The in vitro antiplasmodial activity might be due to the presence of alkaloids, glycosides, flavonoids, phenols, saponins, triterpenoids, proteins, and tannins. In vitro and in vivo studies on antimalarial activity of commiphora. Pdf in vitro antimalarial activity of medicinal plant. The present study investigated the in vitro antiprotozoal activity of sixteen selected medicinal plants. Antiplasmodial activity of botanical extracts against plasmodium falciparum article pdf available in parasitology research 1085. Thus, the present study aims at evaluating the invivo antiplasmodial activity of solvent fractions of methanolic root extract of. In vitro antimalarial activity and cytotoxicity of. Against the drugresistant k1 strain, showed good activity, while 15 showed moder ate antiplasmodial activity. Eleven crude extracts prepared from seven different plant species from western cameroon were screened in vitro for their antiplasmodial activity and cytotoxicity.
The antiplasmodial, antitrypanosomal and antileishmanial activity of 25 plant extracts obtained from seven tanzanian medicinal plants. In order to assess their acclaimed potentials, eleven extracts were prepared from seven selected plants commonly used in western cameroon, and tested both for their antiplasmodial activity and cytotoxicity. Lipophilic extracts of piper hispidum, siparuna andina, s. In vitro assessment of antiplasmodial activity and. In vivo antimalarial activity of extracts of tanzanian medicinal plants. It is not known whether the in vitro effect of these extracts against f32 p. In vitro antiplasmodial activity and cytotoxicity of. In addition, the haemolytic activity tests of the extracts did not reveal haemolytic activity which could interfere with the antimalarial activity. The aim of this study was to evaluate extracts from two medicinal plants, acanthospermum hispidum and ficus thonningii, used in traditional medicine in congo brazzaville, for in vitro antiplasmodial activities against two laboratory strains of plasmodium falciparum. The antiplasmodial activity of the extracts was assessed on cultured p. It is expressed as decreased of parasitaemia levels and percent of inhibition. Ten plants indigenous to sudan and of common use in sudanese folkmedicine, were examined in vitro for antimalarial activity against schizonts maturation of plasmodium falciparum, the major human malaria parasite. Antiplasmodial activity of the ethnobotanical plant cassia. These data provide a scientific rationale for the traditional use of these plants against malaria symptoms.
One of the potential sources of antimalarial drugs is traditional medicinal plants. Medicinal plants play a key role in malaria control in africa, especially in remote areas where health facilities are limited. In vitro antiplasmodial effect of ethanolic extracts of. Table 2 presents the results of antiplasmodial activity and cytotoxicity of the ten extracts tested together with the activity of chloroquine phosphate. The folkloric use of medicinal plants for the management of malaria is well documented. Antiplasmodial activity of diospyros monbuttensis and.
This knowledge of antiplasmodial activity of agnps based on the reported lc 50 values would be helpful in understanding their antimalarial properties as well. The result obtained from our study is higher than most antiplasmodial activity of plantbased silver nanoparticles documented in the previous studies 49, 73, 74. Total antioxidant activity was evaluated using a free radical scavenging assay. Boiled extracts derived from 28 indonesian medicinal plants were screened for their antibabesial activity against babesia gibsoni in vitro. Ellagic acid and the new compound 3,4,5trimethoxyphenyl6ogalloylo. Seven of the eleven extracts exhibited significant antiplasmodial activity ic 50 free online library. Antiplasmodial activity of seven plants used in african. Summary the in vitro antiplasmodial activities of 14 plant species traditionally used in central america for the treatment of malaria or fever were evaluated. Current experimental approach allowed to identify plant extracts with good anti plasmodial activities and to validate their use in the traditional ivorian pharmacopoeia for the treatment of malaria. In vitro antiplasmodial activity and toxicity assessment of plant.
Brine shrimp toxicity and antiplasmodial activity of five. Antimalarial efficacy and toxicological assessment of extracts of. Screening of indonesian medicinal plant extracts for. Crude extracts, fractions and isolated compound were evaluated for antiplasmodial activity against. In the present work, we have studied the antiplasmodial activity of the agnps synthesized from the combination of neem and tulsi plant leaf extracts when taken in different proportions. The agnps synthesized by novel combination of neem and tulsi plant leaf extracts have proved to be potential antiplasmodial agents. Read antiplasmodial activity and toxicity of crude extracts from alternatives parts of plants widely used for the treatment of malaria in burkina faso. Plantbased synthesis of silver nanoparticles using. In this study, we screened extracts of the nine plants for larvicidal, antipyretic, antioxidant and antiplasmodial activity. Evaluation of the in vitro antiplasmodial, antileishmanial, and antitrypanosomal activity of medicinal. Bioassayguided fractionation of the fruit extract of br. Flavonoid versus artemisinin antimalarial activity in. In vitro antiplasmodial activity of some medicinal plants.
In vitro antiplasmodial activity of the plant extracts was done in 96 well plates. Our findings on antiplasmodial activity of these two plants justify the traditional use by local populations against. Antiplasmodial activity of ethanolic extract of macaranga. Promising antiplasmodial activity was found in the extracts from two plants, phyllanthus emblica leaf 50% inhibitory concentration ic 3d7. The plant extracts analysed in this study presented an average antiplasmodial activity 58%. Screening of some extracts from garcinia parvifolia miq. In vivo, malaria, medicinal plants, plasmodium berghei. Pdf in vitro antiplasmodial activity of extract and. Annickia enantia kummeriae annonaceae, artemisia annua asteraceae, pseudospondias microcarpa anacardiaceae, drypetes natalensis euphorbiaceae, acridocarpus chloropterus malpighiaceae, maytenus senegalensis celastraceae and.
The extract demonstrated a dosedependent curative activity at the tested doses 100, 200 and 400. In this work, we studied the in vitro antiplasmodial activity of chloromethylenic, methanolic, and meohh2o 11 crude extracts and decoction obtained from eight medicinal plants collected in burkina faso and of total alkaloids for five plants. In vitro antiplasmodial activity and toxicity assessment. For the plants that showed the stronger antiplasmodial activity a.
Fifteen extracts from nine selected argentine medicinal plants were tested for their antiplasmodial activity in vitro by assessing their ability to inhibit the uptake of 3hhypoxanthine into the. Antiplasmodial activity of plant extracts used in west. The extracts were weighed and dissolved in absolute methanol to obtain a stock solution of 100 mgml. In vivo evaluation of antiplasmodial activity of hydroethanolic stem. Request pdf antiplasmodial activity of plant extracts used in west african traditional medicine five plants originating from ivory coast were selected after an ethnobotanical survey, alchornea. The plant extracts were tested for in vitro activity against a plasmodium falciparum strain d10 using the parasite lactate dehydrogenase pldh assay. The crude extract and fractions also exerted prominent activity against both. All plant samples displayed various antiplasmodial activity. The crude extracts of these plant parts were screened for antiplasmodial, antioxidant, cytotoxic and antibacterial activities.
Antimalarial efficacy and toxicological assessment of extracts of some. Antiplasmodial activity and toxicity of crude extracts. Gentle heart puncture from donor mouse and anesthetized with chloroform afforded 1 ml blood the rising. Antimalarial and antiplasmodial activity of husk extract. Plant materials were extracted with methanol and screened in vitro against erythrocytic schizonts of plasmodium falciparum, intracellular amastigotes of leishmania infantum and trypanosoma cruzi and free trypomastigotes of t. Antiplasmodial activity was found in the extracts of chrozophora oblongifolia, ficus ingens, lavandula dentata and plectranthus barbatus. Dry powdered plant materials were extracted by cold macerations using different solvents. In vitro antiplasmodial and cytotoxic activity of the plant extracts studied.
The two most active extracts that produced 100% inhibition of the parasite growth at concentration of plant material. This study revealed for the first time the antiplasmodial activity of the plant c. Antiplasmodial activity can also be identified by the life cycle inhibition of plasmodium. Antiplasmodial activity of aqueous leaf extract of cymbopogon citratus against plasmodium falciparum infected rats. From the studies conducted on the plant until now, it is known that the methanolic root extract of the plant has schizonticidal activity as high as 84. Antiplasmodial activity of norcaesalpin d and extracts of. In vitro antiplasmodial activity of extract and constituents from esenbeckia febrifuga, a plant traditionally used to treat malaria in the brazilian amazon. Dichloromethane extracts of tetrapleura tetraptera and copaifera religiosa, two plants used to treat malaria in gabon, had interesting antiplasmodial activity in vitro. In vivo validation of antimalarial activity of crude. Antiplasmodial activity of solvent fractions of methanolic. Antiplasmodial activity of vernonia adoensis aqueous. Chloroquinesensitive 3d7 pf3d7 and resistant indo pfindo strains of p. Antiplasmodial activity of methanolic leaf extract of.
In vitro assessment of antiplasmodial activity and cytotoxicity of. Pdf in vitro assessment of the antiplasmodial activity. The antioxidant activity of the plant extracts using the scavenging activity of the stable 1,1 diphenyl 2 picrylhydrazyl ddph free. Pdf antiplasmodial activity and phytochemical analysis of extracts. In vitro antiplasmodial activity of central american. Antigiardial activity of the aqueous extracts of cymbopogon citratus leaves lemongrass and pulicaria undulata herb in comparison with metronidazole, in vitro and in vivo. The antioxidant activity of the plant extracts was assessed using the stable 2,2diphenyl1picrylhydrazyl. The antimalarial activity of extracts was defined according to the ic 50 values obtained.
Antiprotozoal activity and cytotoxicity ic 50 gml of the methanolic extracts of the investigated plants. Pdf antiplasmodial activity and phytochemical analysis. In this study we have attempted to assess the rate of hemolysis with different plant extracts and their fractions. In vitro antiplasmodial activity of crude extracts from togolese medicinal plants article pdf available in asian pacific journal of tropical medicine 42. Of the 4 species assayed, 49% showed promising antiplasmodial activity ic50. Wells with parasitized red blood cells and without plant extract. As a consequence, the present study was carried out in order, first, to identify the. Antiplasmodial, antiinflammatory and cytotoxic activities of various plant extracts from the mascarene archipelago. Antiplasmodial activity was observed from mice blood treated by various concentration of either extract or isolate and the parasitaemia percentage were determined by calculating infected blood cell after 24 h of the treatment.